Protein quantification Mammalian cells

Get tips on using pTY- α-amylase to perform Protein Expression Prokaryotic cells - E. coli Pyrococcus woesei Hyperthermophile α-Amylase

Get tips on using pST44-yAda3Δ2HIS-yAda2Δ1-yGcn5 to perform Protein Expression Prokaryotic cells - E. coli Ada2/Ada3/Gcn5 complex

Get tips on using pHIL‐S1‐opt‐RABV‐G to perform Protein Expression Eukaryotic cells - P. pastoris opt‐RABV‐G

Get tips on using pET-Sac-Aβ(M1–42) to perform Protein Expression Prokaryotic cells - E. coli Aβ(M1–42)

Get tips on using pET-21b(+)/Pro j 1 to perform Protein Expression Prokaryotic cells - E. coli Pro J 1

Get tips on using His-Strep pQE-TriSystem Vector Set to perform Protein Expression Prokaryotic cells - E. coli Integrin αV

Get tips on using pFastBac1- B/Brisbane/60/2008-NP to perform Protein Expression Eukaryotic cells - S. frugiperda Influenza NP

Get tips on using pcDNA™3.1D/V5-His TOPO®-hsEH to perform Protein Expression Eukaryotic cells - HEK293 hsEH

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.