RNA sequencing Rat

Get tips on using Chromatin Immunoprecipitation (ChIP) Assay Kit to perform ChIP Rat - Liver

Get tips on using Pierce™ Magnetic ChIP Kit to perform ChIP Rat - Pancreas

Get tips on using Chromatin Immunoprecipitation (ChIP) Assay Kit to perform ChIP Rat - Pancreas

Get tips on using Imprint® Chromatin Immunoprecipitation Kit to perform ChIP Rat - Brain

Get tips on using Human SCF ELISA Kit (ab100636) to perform ELISA Rat - SC

Get tips on using HSP70 High Sensitivity ELISA kit to perform ELISA Rat - HSP70

Get tips on using Total BDNF Quantikine ELISA Kit to perform ELISA Rat - BDNF

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

Microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.