CRISPR Mouse Deletion ES (embryonic stem) cells

Get tips on using hCas9 to perform CRISPR Mouse - Deletion Dck

Get tips on using NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® to perform RNA sequencing Mouse - ESCs (Embryonic Stem Cells)

Get tips on using hCas9 to perform CRISPR Mouse - Deletion C2C12 Dmd

Get tips on using hCas9 to perform CRISPR Mouse - Deletion B117P Dck

Get tips on using pCas9_GFP to perform CRISPR Mouse - Deletion ANS4 Rosa26

Get tips on using JDS246 to perform CRISPR Mouse - Deletion C2C12 Prnp

Stem cells have the unique ability to self-renew or differentiate themselves into various cell types in response to appropriate signals. These cells are especially important for tissue repair, regeneration, replacement, or in the case of hematopoietic stem cells (HSCs) to differentiate into various myeloid populations. Appropriate signals refer to the growth factor supplements or cytokines that mediate differentiation of various stem cells into the required differentiated form. For instance, HSCs can be differentiated into dendritic cells (with IL-4 and GM-CSF), macrophages (with m-CSF) and MDSCs (with IL-6 and GM-CSF). Human pluripotent stem cells (hPSCs) and induced pluripotent stem cells (iPSCs) can be first cultured in neural differentiation media (GSK3𝛃-i, TGF𝛃-i, AMPK-i, hLIF) to form neural rosettes, which can be differentiated into neural or glial progenitors (finally differentiated into oligodendrocytes). Neural progenitors can be finally differentiated into glutaminergic (dibytyryl cAMP, ascorbic acid) and dopaminergic (SHH, FGF-8, BDNF, GDNF, TGF-𝛃3) neurons. Thus, it is important to first identify the self-renewing cell line: its source and its final differentiation state, followed by the supplements and cytokines required for the differentiation, and final use. Timelines are another thing that is considered. For instance, it takes 7-10 days to form neural rosettes from iPSCs and 3 days to differentiate neural progenitors to neurons. Finally, the stability for stem cell culture media varies. It is advised to make fresh media every time when differentiating HSCs to myeloid populations, whereas neural differentiation media may remain stable for two weeks when stored in dark between 2-8C.

Get tips on using MLM3636 to perform CRISPR Mouse - Deletion Neuro 2a Prnp

Get tips on using JDS246 to perform CRISPR Mouse - Deletion Neuro 2a Prnp

Get tips on using lentiCRISPR to perform CRISPR Mouse - Deletion RAW 264.7 Tfeb