ELISA Human

Get tips on using Human/Mouse/Rat Total HSP70/HSPA1A DuoSet IC ELISA to perform ELISA Mouse - HSP70

Get tips on using Human/Mouse/Rat Total HSP70/HSPA1A DuoSet IC ELISA to perform ELISA Rat - HSP70

Get tips on using Human RBP4/Retinol Binding Protein 4 PicoKine™ ELISA Kit to perform ELISA Human - RBP4

Get tips on using Human/Mouse/RatTotal SOD2/Mn-SOD DuoSet IC ELISA to perform ELISA Rat - SOD2/Mn-SOD

Get tips on using Human Chitinase 3-like 1/YKL-40 PicoKine™ ELISA Kit EK0974 to perform ELISA Human - Chitinase-3-Like Protein-1 (CHI3L1) or YKL-40

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using HSP70 High Sensitivity ELISA kit to perform ELISA Human - HSP70

Get tips on using Mouse GDNF ELISA Kit (ab171178) to perform ELISA Human - GDNF

Get tips on using BMP-2 Quantikine ELISA Kit to perform ELISA Human - BMP-2

Get tips on using Cytochrome c Profiling ELISA Kit (ab110172) to perform ELISA Human - Cytochrome C