crispr-mouse-activation-3t3-l1-c-ebp

Get tips on using pLKO.1 puro to perform CRISPR Mouse - Activation RAW 264.7 Dmd

Get tips on using FxCycle™ PI/RNase Staining Solution to perform Cell cycle assay mouse - 3T3-L1

Get tips on using GeneArt™ CRISPR Nuclease Vector with OFP Reporter Kit to perform CRISPR Human - Activation CD20

Get tips on using GeneArt™ CRISPR Nuclease Vector with OFP Reporter Kit to perform CRISPR Human - Activation ERBB2

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using Rat/Mouse Cytochrome c Quantikine ELISA Kit to perform ELISA Rat - Cytochrome c

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using Mouse C Reactive Protein ELISA Kit (PTX1) (ab157712) to perform ELISA Mouse - C-Reactive Protein/CRP

Get tips on using pX330-U6-Chimeric_BB-CBh-hSpCas9 to perform CRISPR Mouse - Activation C2C12 Lama1

Get tips on using lenti sgRNA(MS2)_zeo backbone to perform CRISPR Mouse - Activation C2C12 FST