Site Directed Mutagenesis (SDM) Human Point mutation THP-1

Get tips on using Human Leptin PicoKine™ ELISA Kit to perform ELISA Human - Leptin

Get tips on using Human LBP PicoKine™ ELISA Kit to perform ELISA Human - LBP

Get tips on using Human Fibronectin PicoKine™ ELISA Kit to perform ELISA Human - Fibronectin

Get tips on using Human FABP2/I-FABP DuoSet ELISA to perform ELISA Human - FABP2

Get tips on using Human Total EGFR DuoSet IC ELISA to perform ELISA Human - EGFR

Get tips on using EGFR (Full-length) Human ELISA Kit to perform ELISA Human - EGFR

Get tips on using Human Decorin ELISA Kit (DCN) (ab99998) to perform ELISA Human - Decorin

Get tips on using Human BDNF PicoKine™ ELISA Kit to perform ELISA Human - BDNF

Get tips on using Adiponectin Human ELISA, High Sensitivity (Sandwich) to perform ELISA Human - Adiponectin

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.