rna-isolation-purification-cells-primary-rat-cardiac-fibroblasts

Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Tissue - Human Decidua

Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Tissue - Human Bladder

Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Tissue - Human Adipose

Get tips on using illustra RNAspin Mini Kit to perform RNA isolation / purification Cells - immortalized BRL 3A

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - immortalized BEAS-2B

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - immortalized A2780/DDP

Get tips on using TruSeq RNA Library Prep Kit v2 to perform RNA sequencing Rat - Retinal ganglion cells (RGCs)

Get tips on using PolyATtract® mRNA Isolation Systems to perform RNA isolation / purification Yeast - Coprinus cinereus

Hello! I used Trizol to extract total RNA from in-vitro cultured bacteria (1 X 10^8 cells). After phase separation, I mixed ~0.4 ml of the upper phase which contains RNA with 0.5 mL cold isopropanol. However, the amount of RNA when measured in Nanodrop was very low. In addition, the ratio between 260 and 230 was around 0.1 to 0.5. Is there a chance that my sample was contaminated by the Trizol reagent? When I collected the aqueous phase I made sure to not touch the lower phase. What should I do?

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Cells - immortalized HeLa, CaSki and C33A (Cervical cancer cells)