siRNA / miRNA gene silencing Human 501 Mel and SK Mel 28 FANCD2

- Found 8034 results

ChIP Human - SW480 Experiment

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.

Proteins ChIP Human SW480

DNA Isolation Kit for Cells and Tissues Product

Get tips on using DNA Isolation Kit for Cells and Tissues to perform DNA isolation / purification Cells - Primary cells Human primary keratinocytes

Products Sigma-Aldrich DNA Isolation Kit for Cells and Tissues

DNA methylation profiling Gene specific profiling - HepG2 Constitutive Androstane Receptor (CAR) Experiment

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

DNA DNA methylation profiling Gene specific profiling HepG2 Constitutive Androstane Receptor (CAR)

DNA methylation profiling Gene specific profiling - UMR-106 BMP2 Experiment

DNA DNA methylation profiling Gene specific profiling UMR-106 BMP2

DNA methylation profiling Gene specific profiling - RH30 rhabdomyosarcoma IGF2 Experiment

DNA DNA methylation profiling Gene specific profiling RH30 rhabdomyosarcoma IGF2

DNA methylation profiling Gene specific profiling - MCF-7 BMP2 Experiment

DNA DNA methylation profiling Gene specific profiling MCF-7 BMP2

DNA methylation profiling Gene specific profiling - Caco-1 MUC1 Experiment

DNA DNA methylation profiling Gene specific profiling Caco-1 MUC1

DNA methylation profiling Gene specific profiling - Caco-1 CHST11 Experiment

DNA DNA methylation profiling Gene specific profiling Caco-1 CHST11

DNA methylation profiling Gene specific profiling - Caco-1 Bax Experiment