siRNA / miRNA gene silencing Mouse Embryonic stem cells

Get tips on using GM-CSF Mouse ELISA Kit to perform ELISA Mouse - GM-CSF

Get tips on using Mouse GM-CSF DuoSet ELISA to perform ELISA Mouse - GM-CSF

Get tips on using Mouse Dkk-1 ELISA Kit to perform ELISA Mouse - Dkk-1

Get tips on using Mouse BMP2 ELISA Kit (ab119582) to perform ELISA Mouse - BMP-2

Get tips on using HiPerFect Transfection Reagent to perform siRNA / RNAi /miRNA transfection Rat - AR42J Lipid based

Get tips on using Mouse SDF1 ELISA Kit (ab100741) to perform ELISA Mouse - SDF-1/CXCL12

Get tips on using Anti-Mouse CD31 (PECAM-1) to perform Immunohistochemistry CD31 - Rat Mouse -NA-

The process of RNA extraction from bacteria, in general, involves an RNA-protective, effective lysis of bacterial cell wall (which may pose difficulties). EDTA promotes loss of outer membrane to provide lysozyme with access to peptidoglycan. Another common method for cell wall lysis is mechanical disruption using a homogenizer (applied for gram-positive bacteria and some strains of gram-negative bacteria). Following lysis, it is necessary to disrupt protein-nucleic acid interactions, which can be achieved by adding sodium dodecyl sulfate (SDS). Next step involves using phenol-chloroform-isoamyl alcohol extraction, where RNA can be obtained from the bottom organic phase, the top phase consists of DNA and the interphase contains proteins. Isoamyl alcohol is an inert and optional addition to this mixture and is added as an anti-foaming reagent to reduce the interphase. Following RNA extraction, the samples should be checked for its quality by gel electrophoresis (23S and 16S rRNAs and 5s rRNA and tRNA bands) or UV spectrophotometric or fluorescence methods.

Get tips on using Rock-2 siRNA and shRNA Plasmids (h) to perform RNA sequencing Human - HT-1376 (urinary bladder cell line)

Get tips on using PE Rat Anti-Mouse CD103 to perform Flow cytometry Anti-bodies Mouse - CD103