Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.
Get tips on using GeneArt™ Site-Directed Mutagenesis PLUS System to perform Site Directed Mutagenesis (SDM) Rat - Point mutation Rat-2 PIK3CB
The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.
Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - Rat Stomach
Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - Rat Spleen
Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Tissue - Rat Nose
Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - Rat Lung
Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - Rat Liver
Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - Rat Kidney
Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - Rat Intestine
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