siRNA / RNAi /miRNA transfection

Get tips on using MISSION® pLKO.1-puro Non-Mammalian shRNA Control Transduction Particles to perform shRNA gene silencing Human - Islets of langerhans Negative control (scrambled) lentiviral particles

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using RNAsimple Total RNA Kit to perform RNA isolation / purification Tissue - Rat Placenta

Get tips on using RNAsimple Total RNA Kit to perform RNA isolation / purification Cells - immortalized H1299

Get tips on using RNAsimple Total RNA Kit to perform RNA isolation / purification Cells - immortalized MA-104

Get tips on using TruSeq Stranded mRNA to perform RNA sequencing Mouse - C2C12

Get tips on using TruSeq Stranded mRNA to perform RNA sequencing Human - H9