Select cell type

Protein isolation

- Found 4660 results

What DNA isolation kit would work for insect samples? Discussion

Hello everyone! I am currently using different DNA isolation kits to extract DNA from insects. Even though I am able to successfully extract DNA I would like to maximize the yield. Do you have any tips that might help me with that even if the kits are not specifically designed for insect samples?

Discussions What DNA isolation kit would work for insect samples?

RNA isolation / purification Tissue - Livestock Blood / Serum / Plasma / Buffy coat Experiment

RNA RNA isolation / purification Tissue Livestock Blood / Serum / Plasma / Buffy coat

RNA isolation from tissue Discussion

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussions RNA isolation from tissue

DNA isolation / purification Cells - Immortalized cell lines Human Neuroblastoma Cell Lines Experiment

DNA DNA isolation / purification Cells Immortalized cell lines Human Neuroblastoma Cell Lines

RNA isolation / purification Cells - Cancer cell lines Leukemia cancer cell lines THP-1 Experiment

RNA RNA isolation / purification Cells Cancer cell lines Leukemia cancer cell lines THP-1

RNA isolation / purification Cells - Cancer cell lines Leukemia cancer cell lines KG-1 Experiment

RNA RNA isolation / purification Cells Cancer cell lines Leukemia cancer cell lines KG-1

Some help with RNA isolation using Trizol Discussion

Hello! I used Trizol to extract total RNA from in-vitro cultured bacteria (1 X 10^8 cells). After phase separation, I mixed ~0.4 ml of the upper phase which contains RNA with 0.5 mL cold isopropanol. However, the amount of RNA when measured in Nanodrop was very low. In addition, the ratio between 260 and 230 was around 0.1 to 0.5. Is there a chance that my sample was contaminated by the Trizol reagent? When I collected the aqueous phase I made sure to not touch the lower phase. What should I do?

Discussions Some help with RNA isolation using Trizol

Prestained Protien ladder Product

Get tips on using Prestained Protien ladder to perform Protein Ladder Prestained

Products HiMEDIA Prestained Protien ladder

pET-32c Product

Get tips on using pET-32c to perform Protein Expression Prokaryotic cells - E. coli rpf-like protein

Products R Verma, Bacteriology and Mycology Division, Mycobacteria Labora pET-32c

EMBacY#5 Product

Get tips on using EMBacY#5 to perform Protein Expression Eukaryotic cells - Hi5 Soluble G protein (Hendra Virus)

Products Sabine Suppmann, Max-Planck Institute of Biochemistry EMBacY#5

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

Outsource experiment