rna-isolation-purification-cells-primary-mouse-dorsal-root-ganglion-neurons

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A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Cellular assays Apoptosis assay cell type T-cells Mouse (OT-I)

Get tips on using illustra RNAspin Mini Kit to perform RNA isolation / purification Cells - immortalized BRL 3A

Products GE Healthcare Life Sciences illustra RNAspin Mini Kit

Get tips on using Absolutely RNA FFPE Kit to perform RNA isolation / purification Tissue - Rat Kidney

Products Agilent Technologies Absolutely RNA FFPE Kit

Get tips on using NucleoSpin® RNA/Protein to perform RNA isolation / purification Tissue - Rat Hippocampus

Products Macherey Nagel NucleoSpin® RNA/Protein

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - immortalized BEAS-2B

Products Qiagen miRNeasy Mini kit

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - immortalized A2780/DDP

Products Qiagen miRNeasy Mini kit

Get tips on using PolyATtract® mRNA Isolation Systems to perform RNA isolation / purification Yeast - Coprinus cinereus

Products Promega PolyATtract® mRNA Isolation Systems

Get tips on using 7ml p53 Bond RTU Primary to perform Immunohistochemistry Human - p53

Products Leica 7ml p53 Bond RTU Primary

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Cells - immortalized HeLa, CaSki and C33A (Cervical cancer cells)

Products Qiagen RNeasy Plus Mini Kit

Get tips on using TRI Reagent® Sigma to perform RNA isolation / purification Cells - immortalized DG-75

Products Sigma-Aldrich TRI Reagent® Sigma

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