DNA methylation profiling Gene specific profiling Mouse muscle stem cells

Get tips on using YeaStar™ Genomic DNA Kit to perform DNA isolation / purification Yeast - Pichia pastoris

Get tips on using YeaStar™ Genomic DNA Kit to perform DNA isolation / purification Yeast - Saccharomyces cerevisiae

Get tips on using Purified Mouse Anti-Nucleoporin p62 Clone 53/Nucleoporin p62 to perform Autophagy assay cell type - N27 dopaminergic cells

Get tips on using PE anti-mouse/rat CD29 Antibody to perform Flow cytometry Anti-bodies Mouse - CD29/β1-Integrin

Get tips on using ScriptSeq Complete Kit (Human/Mouse/Rat) to perform RNA sequencing Mouse - Bone marrow-derived macrophages (BMDMs)

Get tips on using Gibco™DMEM/F-12 to perform Stem cell Differentiation media hUMSCs differentiation into steroidogenic cells

Get tips on using Purified anti-mouse Ly-6C Antibody to perform Flow cytometry Anti-bodies Mouse - Ly6C/Gr-1/Ly6G

Get tips on using Purified anti-mouse Ly-6G Antibody to perform Flow cytometry Anti-bodies Mouse - Ly6C/Gr-1/Ly6G

Get tips on using PE Rat Anti-Mouse Ly-6G to perform Flow cytometry Anti-bodies Mouse - Ly6C/Gr-1/Ly6G

miRNA is the inherent gene silencing machinery which can have more than one mRNA target, whereas siRNA can be designed to target a particular mRNA target. By design, both siRNA and miRNA are 20-25 nucleotides in length. The target sequence for siRNAs is usually located within the open reading frame, between 50 and 100 nucleotides downstream of the start codon. There are two ways in which cells can be transfected with desired RNAi: 1. Direct transfection (with calcium phosphate co-precipitation or cationic lipid mediated transfection using lipofectamine or oligofectamine), and 2. Making RNAi lentiviral constructs (followed by transformation and transduction). Lentiviral constructs are time consuming, but provide a more permanent expression of RNAi in the cells, and consistent gene silencing. Direct transfection of oligonucleotides provides temporary genetic suppression. Traditional methods like calcium phosphate co-precipitation have challenges like low efficiency, poor reproducibility and cell toxicity. Whereas, cationic lipid-based transfection reagents are able to overcome these challenges, along with applicability to a large variety of eukaryotic cell lines. When using oligos, the ideal concentration lies between 10-50nM for effective transfection.