Protein Expression Eukaryotic cells Iranian lizard Leishmania cells

Get tips on using B-PER™ II Bacterial Protein Extraction Reagent (2X) to perform Protein isolation Bacteria - Synechococcus elongatus

Get tips on using B-PER™ II Bacterial Protein Extraction Reagent (2X) to perform Protein isolation Bacteria - Salmonella enterica

Get tips on using B-PER™ II Bacterial Protein Extraction Reagent (2X) to perform Protein isolation Bacteria - Pseudomonas aeruginosa

Get tips on using B-PER™ II Bacterial Protein Extraction Reagent (2X) to perform Protein isolation Bacteria - Escherichia coli

Get tips on using Rat CRP/C Reactive Protein PicoKine™ ELISA Kit to perform ELISA Rat - C-Reactive Protein/CRP

Get tips on using Human CRP/C Reactive Protein PicoKine™ ELISA Kit to perform ELISA Human - C-Reactive Protein/CRP

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using AllPrep DNA/RNA/Protein Mini Kit (50) to perform DNA isolation / purification Tissue - brain