protein-expression-and-purification-mammalian-cells-hek-293-mt-pa

Get tips on using IRIS11 Prestained Protein Ladder to perform Protein Ladder Prestained

Get tips on using Customized Prestained Protein Ladder to perform Protein Ladder Prestained

Get tips on using BlueEasy Prestained Protein Marker to perform Protein Ladder Prestained

Get tips on using Prestained Natural Protein Standards to perform Protein Ladder Prestained

Get tips on using BLUltra Prestained Protein Ladder to perform Protein Ladder Prestained

Get tips on using BlueRAY Prestained Protein Ladder to perform Protein Ladder Prestained

Get tips on using BLUelf Prestained Protein Ladder to perform Protein Ladder Prestained

Get tips on using BlueAQUA Prestained Protein Ladder to perform Protein Ladder Prestained

Get tips on using BLUeye Prestained Protein Ladder to perform Protein Ladder Prestained

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.