Immunohistochemistry Anti-Glial Fibrillary Acidic Protein (GFAP) Mouse

Get tips on using Accell Mouse Nrep (27528) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Mouse - 3T3-L1 P311/Nrep

Get tips on using IntestiCult™ Organoid Growth Medium (Mouse) to perform Stem cell culture media Mouse intestinal stem cells/organoids

Get tips on using APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU to perform TUNEL assay cell type - SK-MEL-2 human melanoma

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.

Get tips on using RIPA Buffer to perform Protein isolation Mammalian cells - Mouse Epididymal fat

Get tips on using Color Prestained Protein Standard, Broad Range (10-250 kDa) to perform Protein Ladder Prestained

Get tips on using Blu13 (BLUelf) Prestained Protein Ladder（5 to 245 kDa） to perform Protein Ladder Prestained

Get tips on using Blu12 (BLUeye) Prestained Protein Ladder（11 to 245 kDa） to perform Protein Ladder Prestained

Get tips on using Blu11 (BlueAQUA) Prestained Protein Ladder（10 to 180 kDa） to perform Protein Ladder Prestained