An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.
Get tips on using CD163 Antibody, anti-human, PE-Vio® 770, REAfinity™ to perform Flow cytometry Anti-bodies Human - CD163
Get tips on using Alexa Fluor® 488 anti-human CD127 (IL-7Rα) Antibody to perform Flow cytometry Anti-bodies Human - CD127
Get tips on using Alexa Fluor® 488 anti-human CD15 (SSEA-1) Antibody to perform Flow cytometry Anti-bodies Human - CD15
Get tips on using EasySep™ Human CD33 Positive Selection Kit II to perform Cell Isolation Monocyte
Get tips on using Monoclonal Mouse Anti-Human Hepatocyte (Concentrate) Clone OCH1E5 to perform Immunohistochemistry Mouse - Hepatocyte
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