rna-isolation-purification-cells-immortalized-ntera-2

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

Get tips on using Rock-2 siRNA and shRNA Plasmids (h) to perform siRNA / RNAi /miRNA transfection Human Cells - HT-1376 ROCK2

Get tips on using Easy-Spin (DNA free) Total RNA Extraction Kit to perform RNA isolation / purification Bacteria - Gram negative Vibro parahaemolyticus

Get tips on using AllPrep DNA/RNA FFPE Kit to perform DNA isolation / purification Tissue - FFPE samples

Get tips on using MagAttract PowerMicrobiome DNA/RNA Kit to perform DNA isolation / purification Micorbiome - Fecal sample

Get tips on using AllPrep DNA/RNA Mini Kit to perform DNA isolation / purification Tissue - fecal sample

Get tips on using Ambion™ RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE to perform RNA isolation / purification Tissue - human kidney tissue

Get tips on using Cox-2 siRNA (m) to perform siRNA / miRNA gene silencing Mouse - B16-F10 COX-2

Get tips on using Dlx-2 siRNA (h) to perform siRNA / miRNA gene silencing Human - MDA-MB-231 DLX-2

Get tips on using Bcl-2 siRNA (h) to perform siRNA / miRNA gene silencing Human - MDA-MB-231 bcl-2