ChIP acH4 Mouse Rabbit

Get tips on using TruSeq RNA Library Prep Kit v2 to perform RNA sequencing Mouse - Microglia

Get tips on using TruSeq RNA Library Prep Kit v2 to perform RNA sequencing Mouse - RAW264.7

Get tips on using GATA-4 Antibody (G-4): sc-25310 to perform Immunohistochemistry Mouse - Gata4

Get tips on using Smooth Muscle Actin Antibody (B4): sc-53142 to perform Immunohistochemistry Mouse - SMA

Get tips on using Anti-alpha smooth muscle Actin antibody (ab5694) to perform Immunohistochemistry Mouse - SMA

Get tips on using Anti-CD133 antibody - Stem Cell Marker (ab19898) to perform Immunohistochemistry Mouse - CD133

Get tips on using Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker to perform Immunohistochemistry Mouse - p62

Get tips on using Recombinant Anti-Lysozyme antibody [EPR2994(2)] (ab108508) to perform Immunohistochemistry Mouse - Lysozyme

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary mouse tracheal epithelial cells