Microarray Gene expression arrays Mouse brain tissue

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Get tips on using Mouse BDNF PicoKine™ ELISA Kit to perform ELISA Mouse - BDNF

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary rat brain microvascular endothelial cells

Get tips on using Neural Maintenance-XF Medium to perform 3D Cell Culture Media Human blood-brain barrier organoid

Get tips on using Gibco™Neurobasal™ Medium to perform 3D Cell Culture Media hESC-derived brain organoids

Get tips on using Gibco™Neurobasal™ Medium to perform 3D Cell Culture Media hiPSC-derived brain organoids

Get tips on using Gibco DMEM/F-12, HEPES to perform 3D Cell Culture Media hiPSC-derived brain organoids

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.