rna-isolation-purification-cells-primary-mouse-dorsal-root-ganglion-neurons

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NucleoSpin® RNA/Protein Product

Get tips on using NucleoSpin® RNA/Protein to perform RNA isolation / purification Tissue - Human Cornea

Products Macherey Nagel NucleoSpin® RNA/Protein

Microarray RNA amplification & Labeling - Mouse Myofibers Cy3- or/and Cy5 Experiment

DNA Microarray RNA amplification & Labeling Mouse Myofibers Cy3- or/and Cy5

Absolutely RNA FFPE Kit Product

Get tips on using Absolutely RNA FFPE Kit to perform RNA isolation / purification Tissue - rat kidney tissue

Products Agilent Technologies Absolutely RNA FFPE Kit

High Pure miRNA Isolation Kit Product

Get tips on using High Pure miRNA Isolation Kit to perform RNA isolation / purification Tissue - Human Bone marrow

Products Sigma-Aldrich High Pure miRNA Isolation Kit

Autophagy assay cell type - Mouse aortic endothelial cells (MAECs) Experiment

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Cellular assays Autophagy assay cell type Mouse aortic endothelial cells (MAECs)

PAXgene Tissue RNA/miRNA Kit Product

Get tips on using PAXgene Tissue RNA/miRNA Kit to perform RNA isolation / purification Tissue - Rat Stomach