rna-isolation-purification-cells-primary-rat-cortical-neurons

Get tips on using mirVana™ miRNA Isolation Kit, with phenol to perform RNA isolation / purification Tissue - Human Esophagus

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.

Get tips on using FastLane Cell Probe Kit (200) to perform RNA isolation / purification Cells - immortalized HT-29

Get tips on using FastLane Cell Probe Kit (200) to perform RNA isolation / purification Cells - immortalized BxPC-3

Get tips on using siGENOME Rat Nrp1 siRNA to perform siRNA / miRNA gene silencing Rat - Schwann cells Nrp1

Get tips on using Direct-zol RNA Kits to perform RNA isolation / purification Bacteria - Gram positive Streptococcus pyogenes

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Tissue - Mouse Small intestine

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Tissue - Mouse Fallopian tubes

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Tissue - Mouse Bone marrow

Get tips on using Isolate II RNA Mini Kit to perform RNA isolation / purification Tissue - mouse lung tissue