siRNA / miRNA gene silencing Mouse Glomerular mesangial cells

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Get tips on using Monoclonal Mouse Anti-Human Hepatocyte (Concentrate) Clone OCH1E5 to perform Immunohistochemistry Mouse - Hepatocyte

Products Agilent Technologies Monoclonal Mouse Anti-Human Hepatocyte (Concentrate) Clone OCH1E5

Get tips on using Mouse Retinol Binding Protein 4 ELISA Kit (ab202404) to perform ELISA Mouse - RBP4

Products Abcam Mouse Retinol Binding Protein 4 ELISA Kit (ab202404)

Get tips on using Mouse MCP-1 PicoKine™ Fast ELISA Kit to perform ELISA Mouse - MCP1

Products BosterBio Mouse MCP-1 PicoKine™ Fast ELISA Kit

Get tips on using Mouse CCL2/JE/MCP-1 Quantikine ELISA Kit to perform ELISA Mouse - MCP1

Products R&D Systems Mouse CCL2/JE/MCP-1 Quantikine ELISA Kit

Get tips on using Mouse Epidermal Growth Factor Receptor (EGFR) ELISA Kit to perform ELISA Mouse - EGFR

Products MyBioSource.com Mouse Epidermal Growth Factor Receptor (EGFR) ELISA Kit

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Cellular assays Autophagy assay cell type Mouse embryonic fibroblasts

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Cellular assays Autophagy assay cell type Mouse lung tissue

Get tips on using EpiCult™-B Mouse Medium Kit to perform Stem cell culture media Murine mammospheres

Products STEMCELL technologies EpiCult™-B Mouse Medium Kit

Proteins Immunohistochemistry Mouse CD166 / ALCAM

Proteins Immunohistochemistry Mouse β-Catenin

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