siRNA / miRNA gene silencing Rat RGC-5

Get tips on using pAC154-dual-dCas9VP160-sgExpression to perform CRISPR Human - Activation HIV-1 5′ LTR

Get tips on using pSpCas9(BB)-2A-Puro (PX459) to perform CRISPR Mouse - Deletion L929 SIRT 5

Get tips on using DMEM/F-12 to perform 3D Cell Culture Media PLC/PRF/5 cells-spheres

Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - SNU-5

Get tips on using Guava Cell Cycle Reagent for Flow Cytometry to perform Cell cycle assay human - OVCAR-5

Get tips on using GeneArt™ Site-Directed Mutagenesis PLUS System to perform Site Directed Mutagenesis (SDM) Rat - Point mutation Rat-2 PIK3CB

Get tips on using Low Input Quick Amp Labeling Kits to perform Microarray RNA amplification & Labeling - Fish fundulus heteroclitus Cyanine-3 / Cyanine-5

Get tips on using Dulbecco’s Modified Eagle’s Medium/Ham’s Nutrient Mixture F12 to perform 3D Cell Culture Media PLC/PRF/5 cells-spheres

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

Get tips on using TruSeq RNA Library Prep Kit v2 to perform RNA sequencing Rat - Retinal ganglion cells (RGCs)