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CRISPR Mouse

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Get tips on using MYH9 CRISPR/Cas9 KO Plasmid (h) to perform CRISPR Rat - Deletion PC12 myosin IIA (Myh9)

Products Santa Cruz Biotechnology MYH9 CRISPR/Cas9 KO Plasmid (h)

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

DNA CRISPR Rat Deletion INS-1 832/13 Ep300

Get tips on using JAM-A CRISPR/Cas9 KO Plasmid (h) to perform CRISPR Human - Deletion F11R

Products Santa Cruz Biotechnology JAM-A CRISPR/Cas9 KO Plasmid (h)

Hi everyone! I am planning on floxing mice with CRISPR but I am having trouble deciding which region to target. Do you have any tips on choosing?

Discussions Floxing mice with CRISPR

Get tips on using GeneArt™ CRISPR Nuclease Vector with OFP Reporter Kit to perform CRISPR Human - Activation CD20

Products Thermo Fisher Scientific GeneArt™ CRISPR Nuclease Vector with OFP Reporter Kit

Get tips on using GeneArt™ CRISPR Nuclease Vector with OFP Reporter Kit to perform CRISPR Human - Activation ERBB2

Products Thermo Fisher Scientific GeneArt™ CRISPR Nuclease Vector with OFP Reporter Kit

Get tips on using GeneArt™ CRISPR Nuclease Vector with OFP Reporter Kit to perform CRISPR Human - Deletion FUT8

Products Thermo Fisher Scientific GeneArt™ CRISPR Nuclease Vector with OFP Reporter Kit

Get tips on using GeneArt™ CRISPR Nuclease Vector with OFP Reporter Kit to perform CRISPR Human - Deletion TRIM25

Products Thermo Fisher Scientific GeneArt™ CRISPR Nuclease Vector with OFP Reporter Kit

Get tips on using GeneArt™ CRISPR Nuclease Vector with OFP Reporter Kit to perform CRISPR Human - Deletion DJ-1

Products Thermo Fisher Scientific GeneArt™ CRISPR Nuclease Vector with OFP Reporter Kit

I would like to excise a large strand of DNA and insert a new one using CRISPR. My problem is that my strand will be a little over 1kb and I am not sure if this is going to be a limiting factor. Also, how long should the homology arms be for a region of this size?

Discussions DNA insert using CRISPR

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