ChIP acH3 Human Sheep

Get tips on using Pierce™ Agarose ChIP Kit to perform ChIP Mouse - RAW264.7

Get tips on using Chromatin Immunoprecipitation (ChIP) Assay Kit to perform ChIP Mouse - HT22

Get tips on using ChromaFlash High-Sensitivity ChIP Kit to perform ChIP Rat - Spinal cord

Get tips on using EpiQuik Chromatin Immunoprecipitation (ChIP) Kit to perform ChIP Rat - INS-1

Get tips on using Chromatin Immunoprecipitation (ChIP) Assay Kit to perform ChIP Mouse - Cardiac fibroblasts

Get tips on using TruSeq ChIP Library Preparation Kit to perform ChIP Mouse - Gonadotrope cell lines

Get tips on using Chromatin Immunoprecipitation (ChIP) Assay Kit to perform ChIP Mouse - 3T3-L1 cells

Get tips on using Pierce™ Magnetic ChIP Kit to perform ChIP Mouse - 3T3-L1 cells

Get tips on using High-Sensitivity ChIP Kit (ab185913) to perform ChIP Mouse - MLL-AF9/NrasG12D AML

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product in the experimental, add more DNA to the PCR reaction or increase the number of amplification cycles. Choose an alternate, ChIP-validated antibody if the antibody does not work.