crispr-human-deletion-f11r

Get tips on using GeneArt™ CRISPR Nuclease Vector with CD4 Enrichment Kit to perform CRISPR Mouse - Deletion NIH 3T3 G3BP

Get tips on using GeneArt™ CRISPR Nuclease Vector with CD4 Enrichment Kit to perform CRISPR Mouse - Deletion NIH 3T3 FXR

Get tips on using lentiCRISPR v2 to perform CRISPR Human - Deletion STING exon 5

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W to perform CRISPR Human - Deletion KAT2A

Get tips on using pX330-U6-Chimeric_BB-CBh-hSpCas9 to perform CRISPR Human - Deletion MGAT1

Get tips on using pSpCas9(BB)-2A-GFP (PX458) to perform CRISPR Human - Deletion PKR

Get tips on using pSpCas9(BB)-2A-GFP (PX458) to perform CRISPR Human - Deletion AURKB

Get tips on using pX330-U6-Chimeric_BB-CBh-hSpCas9 to perform CRISPR Human - Deletion ATM

Get tips on using pX330-U6-Chimeric_BB-CBh-hSpCas9 to perform CRISPR Human - Deletion APC