dna-methylation-profiling-gene-specific-profiling-ca-ski-hpv-16

Get tips on using EZ DNA Methylation-Gold Kit to perform PCR Methylation specific PCR - Bacterial DNA

Get tips on using EZ DNA Methylation-Direct Kit to perform PCR Methylation specific PCR - Mammalian DNA

Get tips on using EZ DNA Methylation-Gold Kit to perform PCR Methylation specific PCR - Mammalian DNA

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using IGEPAL® CA-630 to perform Protein isolation Mammalian cells - Human lung fibroblasts

Does anyone know how “strong” the PCR product of methylation specific PCR is? I kept my PCR products at 4C for about 3 weeks and then at room temperature for another week. Will I be able to use them for sequencing?

Get tips on using gRNA_Cloning Vector to perform CRISPR Human - Repression HPV-16 E7

Get tips on using gRNA_Cloning Vector to perform CRISPR Human - Repression HPV-16 E6

Get tips on using PowerPlex® 16 System to perform Cell line authentication Bladder carcinoma cell line T24