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Get tips on using IntestiCult™ Organoid Growth Medium (Mouse) to perform Stem cell culture media Mouse intestinal stem cells/organoids

Get tips on using Color Prestained Protein Standard, Broad Range (10-250 kDa) to perform Protein Ladder Prestained

Get tips on using Blu13 (BLUelf) Prestained Protein Ladder（5 to 245 kDa） to perform Protein Ladder Prestained

Get tips on using Blu12 (BLUeye) Prestained Protein Ladder（11 to 245 kDa） to perform Protein Ladder Prestained

Get tips on using Blu11 (BlueAQUA) Prestained Protein Ladder（10 to 180 kDa） to perform Protein Ladder Prestained

Get tips on using Blu10 (BlueRAY) Prestained Protein Ladder（10 to 180 kDa） to perform Protein Ladder Prestained

Get tips on using Blue Prestained Protein Standard, Broad Range (11-250 kDa) to perform Protein Ladder Prestained

Get tips on using PageRuler™ Prestained Protein Ladder, 10 to 180 kDa to perform Protein Ladder Prestained

Get tips on using EpiCult™-B Mouse Medium Kit to perform 3D Cell Culture Media Mouse primary breast ephitelial cells-Mammospheres

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.