siRNA / miRNA gene silencing Mouse Embryonic stem cells

Get tips on using HiPerFect Transfection Reagent to perform siRNA / RNAi /miRNA transfection Rat - C6 Cationic lipid based

Get tips on using Gibco™DMEM/F-12 to perform Stem cell culture media hMegakaryoblastic cells

Get tips on using Gibco™ RPMI 1640 Medium to perform Stem cell culture media hMegakaryoblastic cells

Get tips on using Gibco™ DMEM, high glucose to perform Stem cell culture media hMyoblasts cells

Get tips on using Gibco™DMEM/F-12 to perform Stem cell culture media hMyoblasts cells

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using Attractene Transfection Reagent to perform shRNA gene silencing Human - HEK 293T CAPN5- (Calpains) cationic lipid based

Get tips on using Lipofectamine® 2000 Transfection Reagent to perform siRNA / RNAi /miRNA transfection Rat - C6 Lipofectamine

Get tips on using Gibco™ StemPro™ hESC SFM to perform Stem cell Differentiation media hiPSCs or hESCs differentiation to Embryoid body (EB)

Get tips on using STEMdiff™ APEL™ 2 Medium to perform Stem cell Differentiation media hiPSCs or hESCs differentiation to Embryoid body (EB)