DNA methylation profiling Gene specific profiling MRC-5

- Found 5468 results

pSpCas9(BB)-2A-Puro (PX459) Product

Get tips on using pSpCas9(BB)-2A-Puro (PX459) to perform CRISPR Mouse - Deletion L929 SIRT 5

Products Addgene pSpCas9(BB)-2A-Puro (PX459)

siRNA / miRNA gene silencing Mouse - Glomerular mesangial cells HIPK2 Polymer / Lipid delivery Experiment

RNA siRNA / miRNA gene silencing Mouse Glomerular mesangial cells HIPK2 Polymer / Lipid delivery

siRNA / miRNA gene silencing Human - Jurkat MK2 (MAPK Kinase 2) Viral vectors Experiment

RNA siRNA / miRNA gene silencing Human Jurkat MK2 (MAPK Kinase 2) Viral vectors

siRNA / miRNA gene silencing Human - U937 MK2 (MAPK Kinase 2) Viral vectors Experiment

RNA siRNA / miRNA gene silencing Human U937 MK2 (MAPK Kinase 2) Viral vectors

DNA isolation / purification Cells - Immortalized cell lines Human Neuroblastoma Cell Lines Experiment

DNA DNA isolation / purification Cells Immortalized cell lines Human Neuroblastoma Cell Lines

DMEM/F-12 Product

Get tips on using DMEM/F-12 to perform 3D Cell Culture Media PLC/PRF/5 cells-spheres

Products Thermo Fisher Scientific DMEM/F-12

CYTO-ID® Autophagy detection kit Product

Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - SNU-5

Products Enzo Life Sciences CYTO-ID® Autophagy detection kit

siRNA/RNAi/miRNA transfection human Discussion

I would like to regulate the expression of a gene and in order to do that, I have purchased specific siRNA. After optimizing my transfection protocol and using electroporation I have achieved a 60-70% reduction of the gene of interest. However, I cannot observe a significant reduction of mRNA expression but only a reduction of protein. What might be the problem? Could the problem be in my cell treatment method?

Discussions siRNA/RNAi/miRNA transfection human

siRNA / miRNA gene silencing Human - HNSCC cell line Eph receptor B4 Polymer / Lipid Experiment

RNA siRNA / miRNA gene silencing Human HNSCC cell line Eph receptor B4 Polymer / Lipid

siRNA / miRNA gene silencing Human - Primary Human Aortic Endothelial Cells GLO-1 Lipid Experiment

RNA siRNA / miRNA gene silencing Human Primary Human Aortic Endothelial Cells GLO-1 Lipid

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