Protein expression and purification Insect cells

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Get tips on using MasterPureTM-Yeast DNA Purification kit to perform DNA isolation / purification Yeast - Pichia pastoris

Products Epicentre MasterPureTM-Yeast DNA Purification kit

Get tips on using MasterPureTM-Yeast DNA Purification kit to perform DNA isolation / purification Yeast - Saccharomyces cerevisiae

Products Epicentre MasterPureTM-Yeast DNA Purification kit

Get tips on using Human RBP4/Retinol Binding Protein 4 PicoKine™ ELISA Kit to perform ELISA Human - RBP4

Products BosterBio Human RBP4/Retinol Binding Protein 4 PicoKine™ ELISA Kit

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Cellular assays Live / Dead assay mammalian cells Spleen cells

Isolating RNA from tissues and paraffin-embedded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the integrity of RNA.

RNA RNA isolation / purification Tissue Human Blood / Serum / Plasma / Buffy coat

Isolating RNA from tissues and paraffin-embedded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the integrity of RNA.

RNA RNA isolation / purification Tissue Mouse Blood / serum / plasma / buffy coat

Isolating RNA from tissues and paraffin-embedded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the integrity of RNA

RNA RNA isolation / purification Tissue Rat Blood / Serum / Plasma / Buffy coat

Get tips on using Total RNA Purification Plus Kit to perform RNA isolation / purification Bacteria - Gram negative Vibro cholerae

Products Norgen Biotek Total RNA Purification Plus Kit

Get tips on using GeneJET Genomic DNA Purification Kit to perform DNA isolation / purification Bacteria - Gram positive Streptomyces. Sp

Products Thermo Fisher Scientific GeneJET Genomic DNA Purification Kit

Get tips on using Prestained Dual Color Protein Molecular Weight Marker (1.7-40 kDa) Molecular Weight Marker to perform Protein Ladder Prestained

Products MyBioSource.com Prestained Dual Color Protein Molecular Weight Marker (1.7-40 kDa) Molecular Weight Marker

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