siRNA / RNAi /miRNA transfection Human Cells KG-1

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Get tips on using Rat IGF-1 PicoKine™ ELISA Kit to perform ELISA Rat - IGF-I

Products BosterBio Rat IGF-1 PicoKine™ ELISA Kit

Flow cytometry is an immunophenotyping technique whereby sing cell suspensions are stained for either cell surface markers or intracellular proteins by fluorescently-labelled antibodies and analyzed with a flow cytometer, where fluorescently-labelled molecules are excited by the laser to emit light at varying wavelengths, which is then detected by the instrument. There are several key criteria which are required to be kept in mind while designing a flow experiment- 1. Antibody titration (optimal dilution of antibodies should be calculated in order to avoid over- or under- saturated signals for proper detection of surface and intracellular markers), 2. Precision (3 or more replicates of the sample should be used per experiment), 3. Specificity (proper isotype controls should be included in the experiment), 4. Day-to-day variability (experiments should be repeated 3 or more times to ensure consistency and avoid variability due to flow cytometer settings), 5. Antibody interaction (Fluorescence minus one or FMO should be used, which is the comparison of signals from panel minus one antibody vs. the full panel), and 6. Antibody stability (fluorescently-labelled antibodies should be stored at 4C).

Proteins Flow cytometry Anti-bodies Mouse Ly6C/Gr-1/Ly6G
Fenozol Product

Get tips on using Fenozol to perform siRNA / miRNA gene silencing Rat - IEC-6 HuR

Products A&A Biotechnology Fenozol

Get tips on using Anti-Collagen I antibody [COL-1] (ab90395) to perform Western blotting Type I collagen

Products Abcam Anti-Collagen I antibody [COL-1] (ab90395)

Get tips on using CD279 (PD-1) Monoclonal Antibody (J43), PE, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD279/PD-1

Products eBioscience CD279 (PD-1) Monoclonal Antibody (J43), PE, eBioscience™

Get tips on using CD31 (PECAM-1) Monoclonal Antibody (390), Biotin, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD31/Pecam-1

Products eBioscience CD31 (PECAM-1) Monoclonal Antibody (390), Biotin, eBioscience™

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

RNA RNA sequencing Rat INS-1 832/13

Get tips on using Mouse Sca-1/Ly6 APC-conjugated Antibody to perform Flow cytometry Anti-bodies Mouse - Ly-6A-E/Sca1

Products R&D Systems Mouse Sca-1/Ly6 APC-conjugated Antibody
JetPrime Product

Get tips on using JetPrime to perform DNA transfection Mammalian cells - Immortalized cell lines HeLa

Products Polyplus transfections JetPrime
JetPrime Product

Get tips on using JetPrime to perform DNA transfection Mammalian cells - Immortalized cell lines COS7

Products Polyplus transfections JetPrime

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