siRNA / miRNA gene silencing Mouse NIH-3T3

Isolating RNA from tissues and paraffin-embedded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the integrity of RNA.

Get tips on using Mouse Sca-1/Ly6 APC-conjugated Antibody to perform Flow cytometry Anti-bodies Mouse - Ly-6A-E/Sca1

Get tips on using Biotin Rat Anti-Mouse Ly-6A/E to perform Flow cytometry Anti-bodies Mouse - Ly-6A-E/Sca1

Get tips on using Lipofectamine® 2000 Transfection Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines 3T3-L1

Get tips on using β-Gal Reporter Gene Assay, chemiluminescent to perform Reporter gene assay β-galactosidase substrates - RAW 264.7

Get tips on using β-Gal Reporter Gene Assay, chemiluminescent to perform Reporter gene assay β-galactosidase substrates - SH-SY5Y

Get tips on using BUV395 Mouse Anti-Human CD123 to perform Flow cytometry Anti-bodies Human - CD123/IL3-R

Get tips on using PE Mouse Anti-Human CD31 to perform Flow cytometry Anti-bodies Human - CD31/PECAM-1

Get tips on using PRO-PREP™ Protein Extraction Solution (C/T) to perform Protein isolation Mammalian cells - 3T3-L1

Get tips on using ROS-ID® Total ROS/Superoxide detection kit to perform ROS assay cell type - 3T3-L1