rna-isolation-purification-cells-immortalized-ntera-2

Get tips on using BLOOD AGAR BASE NO.2 to perform Bacterial cell culture media Helicobacter pylori

Get tips on using EpiTect 96 Bisulfite Kit (2) to perform Bisulfite DNA Modification Fluids

Get tips on using FaqI (BsmFI) (2 U/µL) to perform Restriction Enzymes BsmFI / FaqI

Get tips on using BspPI (AlwI) (2 U/µL) to perform Restriction Enzymes AlwI / BspPI

Get tips on using AllPrep Bacterial DNA/RNA/Protein Kit (50) to perform DNA isolation / purification Bacteria - Gram negative Massilia sp

Get tips on using TIMP-2 Antibody (B-12): sc-365671 to perform Western blotting TIMP-2

Get tips on using Mouse BMP-2 PicoKine™ ELISA Kit to perform ELISA Mouse - BMP-2

Get tips on using Human BMP-2 PicoKine™ ELISA Kit to perform ELISA Human - BMP-2

Get tips on using RIPA Buffer to perform Protein isolation Mammalian cells - SK-N-BE(2)-C

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.