ELISA (kit) IL-6, IL-1β, IL-8 and TNF-α -NA- Human

Get tips on using BD Cycletest™ Plus DNA Kit to perform Cell cycle assay human - BxPC-3

Get tips on using Muse® Cell Cycle Assay Kit to perform Cell cycle assay human - BxPC-3

Get tips on using Muse® Cell Cycle Assay Kit to perform Cell cycle assay human - Hep G2

Get tips on using BD Cycletest™ Plus DNA Kit to perform Cell cycle assay human - Hep G2

Get tips on using Muse® Cell Cycle Assay Kit to perform Cell cycle assay human - PANC-1

Get tips on using PE Annexin V Apoptosis Detection Kit I to perform Apoptosis assay cell type - Human T-cells

Get tips on using FITC Annexin V Apoptosis Detection Kit I to perform Apoptosis assay cell type - Human T-cells

Get tips on using Expi293™ Expression System Kit to perform Protein expression and purification Mammalian cells - HEK 293 HER2 leader peptide

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.