siRNA / RNAi /miRNA transfection Mouse Melanoma cells (B16)

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using MUC2 (MRQ-18) Mouse Monoclonal Antibody to perform Immunohistochemistry Human - Muc-2

Get tips on using Cyclin E1 (HE12) Mouse mAb #4129 to perform Western blotting Cyclin E

Get tips on using Human/Mouse Active Caspase-3 Antibody to perform Western blotting Caspase-3

Get tips on using Rat / Mouse FGF-21 ELISA Kit to perform ELISA Rat - FGF-21

Get tips on using Alexa Fluor® 647 Rat Anti-Mouse CD206 to perform Flow cytometry Anti-bodies Mouse - CD206

Get tips on using PE-Cy™7 Rat Anti-Mouse CD86 to perform Flow cytometry Anti-bodies Mouse - CD86

Get tips on using Alexa Fluor® 700 Rat Anti-Mouse CD86 to perform Flow cytometry Anti-bodies Mouse - CD86

Get tips on using Brilliant Violet 510™ anti-mouse CD69 Antibody to perform Flow cytometry Anti-bodies Mouse - CD69

Get tips on using PerCP-Cy™5.5 Hamster Anti-Mouse CD69 to perform Flow cytometry Anti-bodies Mouse - CD69