Site Directed Mutagenesis (SDM) Human Point mutation Caco-2

Get tips on using MACSprep™ PBMC Isolation Kit, human to perform Cell Isolation PBMC Isolation

Get tips on using Human/Mouse Active Caspase-3 Antibody to perform Western blotting Caspase-3

Get tips on using ScriptSeq Complete Kit (Human/Mouse/Rat) to perform RNA sequencing Mouse - J774

Get tips on using Quantifiler™ Human DNA Quantification Kit to perform DNA quantification Brain tissue

Get tips on using GeneChip® Human Genome U133 Plus 2.0 Array to perform Microarray Gene expression arrays - Rhesus monkey brain tissue Biotin

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

Get tips on using PE Mouse Anti-Human CD61 Clone VI-PL2 to perform Flow cytometry Anti-bodies Human - CD61

Get tips on using PE Mouse Anti-Human CD26 Clone M-A261 to perform Flow cytometry Anti-bodies Human - CD26

Get tips on using PE-Cy™7 Mouse Anti-Human CD10 to perform Flow cytometry Anti-bodies Human - CD10