rna-isolation-purification-cells-primary-mouse-cortical-neurons

Get tips on using AllPrep DNA/RNA Mini Kit to perform RNA isolation / purification Bacteria - Gram positive Bacillus anthracis

Get tips on using ISOLATE II RNA Micro Kit to perform RNA isolation / purification Bacteria - Gram negative Vibro cholerae

Get tips on using DMEM/F-12 to perform 3D Cell Culture Media Mouse primary breast cancer ephitelial cells-Mammospheres

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using EZ1 RNA Tissue Mini Kit (48) to perform RNA isolation / purification Tissue - Human FFPE tissue

Get tips on using InviTrap Spin Tissue RNA Mini Kit to perform RNA isolation / purification Tissue - Human Artery / Aorta

Get tips on using AllPrep DNA/RNA/miRNA Universal Kit to perform RNA isolation / purification Tissue - human lung tissue

Get tips on using AllPrep DNA/RNA/miRNA Universal Kit to perform RNA isolation / purification Tissue - human adipose tissue

Get tips on using mirVana™ miRNA Isolation Kit, with phenol to perform RNA isolation / purification Bacteria - Gram positive Staphylococcus saprophycitius

Get tips on using mirVana™ miRNA Isolation Kit, with phenol to perform RNA isolation / purification Bacteria - Gram negative Klebsiella pneumoniae