Get tips on using PE Rat Anti-Mouse CD103 to perform Flow cytometry Anti-bodies Mouse - CD103
Get tips on using PE Rat Anti-Mouse CD73 to perform Flow cytometry Anti-bodies Mouse - CD73
Get tips on using Purified Hamster Anti-Mouse CD80 to perform Flow cytometry Anti-bodies Mouse - CD80
Get tips on using PerCP Rat Anti-Mouse CD8a to perform Flow cytometry Anti-bodies Mouse - CD8a
Get tips on using Silencer®_Faslg siRNA (r) to perform siRNA / miRNA gene silencing Rat - F98 Faslg
Get tips on using Rn_LOC312647_1_ ATG7 FlexiTube siRNA(r) to perform siRNA / miRNA gene silencing Rat - NRVM( ATG7
Get tips on using Silencer® Select_Alkbh1 siRNA (r) to perform siRNA / miRNA gene silencing Rat - B35 Alkbh1
Get tips on using ON-TARGETplus human ATG16L1 siRNA to perform siRNA / miRNA gene silencing Human - SHSY5Y ATG16L1
Get tips on using ON-TARGETplus Human TET1 siRNA to perform siRNA / miRNA gene silencing Human - A172 TET1
Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.
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