CRISPR Mouse Deletion ES (embryonic stem) cells

Get tips on using Gibco™Glasgow's MEM (GMEM) to perform Stem cell Differentiation media mESCs differentiation into Neuroepithelial Cyst

Get tips on using Gibco™DMEM/F-12 to perform Stem cell Differentiation media mESCs differentiation into Neuroepithelial Cyst

Get tips on using Dulbecco’s Modified Eagle’s Medium - high glucose to perform Stem cell culture media Rat oligodendrocyte precursors (OPCs)

Get tips on using MEBMTM Mammary Epithelial Cell Growth Basal Medium to perform 3D Cell Culture Media Mouse primary breast ephitelial cells-Mammospheres

Get tips on using LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit to perform Live / Dead assay mammalian cells - mouse, T-cell

Get tips on using SMARTpool: ON-TARGETplus Hipk2 siRNA to perform siRNA / miRNA gene silencing Mouse - Glomerular mesangial cells HIPK2 Polymer / Lipid delivery

Get tips on using CytoTox 96® Non-Radioactive Cytotoxicity Assay to perform Live / Dead assay mammalian cells - mouse bone marrow-derived macrophages

Get tips on using MethylFlash Methylated DNA 5-mC Quantification Kit to perform DNA methylation profiling Whole genome profiling - C2C12 mouse myoblast cells

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using EGMTM -2 MV Microvascular Endothelial Cell Growth Medium-2 BulletKitTM to perform Stem cell culture media hPericytes