ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.
ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.
Get tips on using Oct-3/4 Antibody (C-10): sc-5279 to perform Western blotting Oct4
Get tips on using Apo-ONE® Homogeneous Caspase-3/7 Assay to perform Apoptosis assay cell type - SH-SY5Y
Get tips on using OxiSelect™ Comet Assay Kit (3-Well Slides) to perform DNA Damage Assay HeLa
Get tips on using OxiSelect™ Comet Assay Kit (3-Well Slides) to perform DNA Damage Assay MCF 10A
Get tips on using OxiSelect™ Comet Assay Kit (3-Well Slides) to perform DNA Damage Assay Hep G2
Get tips on using OxiSelect™ Comet Assay Kit (3-Well Slides) to perform DNA Damage Assay HEK 293T
Get tips on using NucleoSpin® RNA/Protein to perform Protein isolation Mammalian cells - Human eutopic endometrial stromal cells
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment