CRISPR Rat Deletion INS-1 832/13

Get tips on using pSB 130 to perform Protein Expression Prokaryotic cells - A. tumefaciens ELP-intein

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

Get tips on using Stealth siRNA_SPI1 to perform siRNA / miRNA gene silencing Human - LAD2 PU.1/SPI1

Get tips on using siRNA Wee1 to perform siRNA / miRNA gene silencing Human - SKOV-3 Wee-1

Get tips on using pASK-TS-MCoTI to perform Protein Expression Prokaryotic cells - E. coli SFTI-1

Get tips on using Hs_IFI16_1 FlexiTube siRNA to perform siRNA / miRNA gene silencing Human - TF‐1 IFI16

Get tips on using pSilencer 2.1-U6 Hygro to perform shRNA gene silencing Human - SHSY5Y Beclin 1

Get tips on using AChE shRNA Plasmids (h) to perform shRNA gene silencing Human - TF‐1 AChE

Get tips on using Gibco™ DMEM, high glucose to perform Mammalian cell culture media PANC-1

Get tips on using Annexin-V-FLUOS Staining Kit to perform Apoptosis assay cell type - THP-1