siRNA / RNAi /miRNA transfection Human Cells HNSCC

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using Human VEGF PicoKine™ ELISA Kit to perform ELISA Human - VEGF

Get tips on using Human Sonic Hedgehog ELISA Kit (ab100639) to perform ELISA Human - ShhN

Get tips on using Human SDC1 / Syndecan-1 ELISA Kit to perform ELISA Human - SDC1

Get tips on using Human Prolactin PicoKine™ ELISA Kit to perform ELISA Human - PRL

Get tips on using Human Osteopontin (OPN) Quantikine ELISA Kit to perform ELISA Human - OPN

Get tips on using Human MCP-1 ELISA Kit (ab179886) to perform ELISA Human - MCP1

Get tips on using Human Myeloperoxidase ELISA Kit (MPO) (ab119605) to perform ELISA Human - MPO

Get tips on using Human MMP-9 Quantikine ELISA Kit to perform ELISA Human - MMP9

Get tips on using Human MMP9 ELISA Kit (lyophilized) (ab100610) to perform ELISA Human - MMP9