siRNA / RNAi /miRNA transfection Mouse Primary Splenocytes

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

Get tips on using Monoclonal Mouse Anti-Human Hepatocyte (Concentrate) Clone OCH1E5 to perform Immunohistochemistry Mouse - Hepatocyte

Get tips on using Mouse Retinol Binding Protein 4 ELISA Kit (ab202404) to perform ELISA Mouse - RBP4

Get tips on using Mouse MCP-1 PicoKine™ Fast ELISA Kit to perform ELISA Mouse - MCP1

Get tips on using Mouse CCL2/JE/MCP-1 Quantikine ELISA Kit to perform ELISA Mouse - MCP1

Get tips on using Mouse Epidermal Growth Factor Receptor (EGFR) ELISA Kit to perform ELISA Mouse - EGFR