rna-isolation-purification-cells-immortalized-saos-2

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Rat Bone

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Rat Adipose

Get tips on using Stat3 Antibody (F-2): sc-8019 to perform Western blotting STAT3

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.

Get tips on using MinElute 96 UF PCR Purification Kit (24) to perform DNA isolation / purification Plasmid purification

Get tips on using E-cadherin monoclonal antibody (ECCD-2) to perform Western blotting E-cadherin

Get tips on using Mouse Lipocalin-2/NGAL DuoSet ELISA to perform ELISA Mouse - NGAL/LCN2

Get tips on using peSpCas9(1.1)-2×sgRNA (empty, donor) to perform CRISPR Human - Repression SLC7A11