siRNA / miRNA gene silencing Mouse Neuro 2a

Get tips on using Mouse/Rat Angiopoietin-2 Quantikine ELISA Kit to perform ELISA Rat - ANGPT2

Get tips on using APC/Cyanine7 anti-mouse I-A/I-E Antibody to perform Flow cytometry Anti-bodies Mouse - MHCII

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

Get tips on using Brilliant Violet 650™ anti-mouse IFN-γ Antibody to perform Flow cytometry Anti-bodies Mouse - IFN-γ

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary mouse cortical neurons

Get tips on using Fibroblast Growth Factor 21 Mouse/Rat ELISA to perform ELISA Rat - FGF-21

Get tips on using Mouse/Rat FGF-21 Quantikine ELISA Kit to perform ELISA Rat - FGF-21

Get tips on using Rat/Mouse Cytochrome c Quantikine ELISA Kit to perform ELISA Rat - Cytochrome c

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.