siRNA / miRNA gene silencing Rat RMC-1

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that no responses other than those related to the signaling pathway of interest. This can be achieved by selecting a highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzyme such as luciferase.

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Get tips on using Senescence β-Galactosidase Staining Kit - Cell Signaling to perform Reporter gene assay β-galactosidase substrates - rat nucleus pulposus

Get tips on using CD80 (B7-1) Monoclonal Antibody (16-10A1), APC, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD80

Get tips on using CD80 (B7-1) Monoclonal Antibody (16-10A1), PE, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD80

Get tips on using Human Chitinase 3-like 1/YKL-40 PicoKine™ ELISA Kit EK0974 to perform ELISA Human - Chitinase-3-Like Protein-1 (CHI3L1) or YKL-40

Get tips on using PrepSEQ™ 1-2-3 Mycoplasma Nucleic Acid Extraction Kit to perform Cell Culture Contamination Detection Kit Mycoplasma

Get tips on using Alexa Fluor® 488 anti-human CD15 (SSEA-1) Antibody to perform Flow cytometry Anti-bodies Human - CD15

Get tips on using TransIT-TKO Transfection Reagent to perform siRNA / RNAi /miRNA transfection Mouse - Primary Splenocytes Polymer / lipid

Get tips on using Lipofectamine® 2000 Transfection Reagent to perform siRNA / RNAi /miRNA transfection Human Cells - HeLa Lipofectamine