shRNA gene silencing Mouse Prostate cancer cell lines (DU145 and PC3) CD24

Get tips on using siGENOME Human BRCA1 (672) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - ES2 BRCA1

Get tips on using siGENOME Human DAB2 (1601) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - A549 DAB2

Get tips on using Accell Human MYB (4602) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - LAMA84 MYB

Get tips on using Accell Human VEGFC (7424) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - LAMA84 VEGFC

Get tips on using Accell Human MYB (4602) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - K562 MYB

Get tips on using Accell Human VEGFC (7424) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - K562 VEGFC

Get tips on using HIF-1α siRNA (r) to perform siRNA / miRNA gene silencing Rat - Brain endothelial cells HIF-1α Lipid

Get tips on using Purified Mouse Anti-Nucleoporin p62 Clone 53/Nucleoporin p62 to perform Autophagy assay cell type - N27 dopaminergic cells

Get tips on using C/EBP β siRNA (r) to perform siRNA / miRNA gene silencing Rat - Glial cells C/EBP‐β

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.